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ILT > News > New Technologies > What is HPLC Chromatography?

What is HPLC Chromatography?

2 January 2018 Posted by: makeyourmark New Technologies

High-performance liquid chromatography (HPLC) is a powerful analytical technique used in a wide range of industries for the separation, detection, and determination of chemical compounds. It is a separation technique used to separate, identify, and quantify the components of a mixture. This powerful and versatile analytical technique offers high resolution, sensitivity, and accuracy in order to identify and quantify individual compounds in a mixture.

HPLC Chromatography Phases

HPLC is a type of liquid chromatography that uses a high-pressure pump to force a solvent (mobile phase) through a column filled with solid particles (stationary phase). The stationary phase is usually a porous material, such as silica, coated with a thin film of a chemical that can interact with the components of the mixture. The components of the mixture then travel through the column at different rates and are separated based on their interaction with the stationary phase.

The mobile phase is usually a mixture of organic solvents such as methanol, acetonitrile, and water, or a combination of these. The choice of solvent depends on the type of sample being analyzed and the type of separation needed. As the mobile phase passes through the column, the different components will interact differently with the stationary phase, causing them to move at different rates and eventually separate. The components can then be detected and quantified using a detector, such as a UV-Vis detector or a mass spectrometer.


HPLC is used for a wide range of applications, including the analysis of pharmaceuticals, food and beverages, petrochemicals, and environmental samples. It is also a popular technique for protein and peptide analysis, due to its high resolution and sensitivity.

This type of liquid chromatography offers high resolution, sensitivity, and accuracy and can be used to analyze a wide range of samples. It is also relatively easy to use and can be used to analyze complex mixtures with ease.

HPLC Vial Caps

HPLC vial caps are essential components in the operation of any liquid chromatography system. They’re typically used to ensure a secure, airtight seal for the vials in which the samples are stored and analyzed. These caps are typically made of a variety of materials, including polypropylene, polyethylene, with liner materials made using high grade silicone faced with PTFE.

HPLC vials and caps are designed to provide a leak-proof seal that prevents any loss of sample or other material during the sample preparation and analysis process. They also help to ensure that the vials remain securely sealed and prevent any contamination from entering the sample. This is especially important for sensitive samples, such as those used for drug development and related research.

In addition to providing a secure seal, they can also be used to help identify the sample. Many caps feature a unique identification code that can be used to easily identify the sample. This can be especially helpful when dealing with large batches of samples or when working with multiple laboratories.

HPLC is an invaluable tool for separating and analyzing complex samples, and it is used in many different fields including the pharmaceutical industry. It is a highly efficient and versatile technique, and it is used to identify, quantify, and monitor a wide variety of components in a sample. HPLC vials, caps, and septa are an important part of this process and having reliable products are vital to these industries.

Read more about the seals and septa industry on our blog page or learn more about ILT, the world leader in manufacturing seals and septa here.