Office: 518-432-0617
Sales Hours no sales hours
Address 45 Discovery Drive, Rensselaer, NY 12144
Fax: 518-432-9146
Sales Hours no sales hours
Address 45 Discovery Drive, Rensselaer, NY 12144

Production Testing Levels

Production Testing Levels

Septa used in chromatography must endure repeated punctures, high temperatures, and exposure to strong chemicals. However, these physical properties are not the only critical factors. The analytical cleanliness of the septa is just as important. At ILT, we’ve developed our own conditioning and testing standards to ensure the quality of our products. These production testing levels are designed to standardize the performance of septa, ensuring labs can choose the right product for their specific needs, whether they’re conducting basic chromatography or more advanced testing that requires low bleed and ultra-low bleed septa.

ILT’s Guide to Production Testing Levels

Level 1

Level 1 products are acceptable for use in gasket, septa, and cap liners for standard chromatography applications. Products are free of contaminants.

Level 2

Level 2 products contain reduced volatiles. These septa are suitable for gas chromatography and liquid chromatography analysis including single injection headspace testing.

Level 3

Level 3 products are low bleed. These septa are suitable for more rigorous testing applications such as EPA method 5035 and multiple puncture headspace testing.

Level 4

Level 4 products are ultra low bleed products. These septa are suitable for use as injection port septa for gas chromatography and any other applications where volatile free septa is required.

The Importance of Volatile Reduction in Gas Chromatography and Headspace Analysis

In gas chromatography (GC) and headspace analysis, the reduction of volatile release from septa is paramount. Septa with high volatile content can introduce contaminants into the sample chamber, leading to inaccurate readings and potentially costly errors. This is especially problematic when conducting headspace analysis, where even a small amount of contamination can disrupt the detection of volatile compounds. Level 2 and 3 septa, which offer reduced volatiles, are commonly used for these applications because they minimize the release of contaminants during testing. This makes them ideal for industries such as food and beverage safety, where labs must detect tiny amounts of volatile organic compounds (VOCs) to ensure product safety and quality.

For labs conducting tests such as EPA Method 5035, which involves multiple punctures during sample collection and analysis, the analytical cleanliness provided by Level 3 septa ensures minimal contamination and reliable results. These septa withstand repeated use, making them a cost-effective choice for high-throughput testing environments.

Materials Used in Septa and Their Impact on Volatile Release

The type of conditioning the material receives directly impacts performance, particularly in terms of volatiles released during use. Septa are typically made from materials like silicone, PTFE, and butyl rubber, each offering distinct advantages depending on the application. For example, silicone/PTFE septa are known for their flexibility and resistance to coring, making them ideal for applications where multiple punctures are required. They also provide superior chemical resistance, making them suitable for handling aggressive solvents. Conditioning these materials to a low level of volatiles is particularly important for applications like gas chromatography. By choosing the right septa conditioning level, labs can ensure the performance of their septa matches their analytical needs while improving the accuracy of their methods.

Applications of Low Bleed and Ultra-Low Bleed Septa in Sensitive Testing

Low bleed and ultra-low bleed septa are essential in sensitive testing environments like pharmaceutical development, forensic toxicology, and environmental analysis. ILT’s Level 3 products are classified as low bleed, minimizing contamination risks in tests where even minimal bleed could interfere with result accuracy. For high-sensitivity tests like mass spectrometry, low bleed septa ensure precise readings. ILT’s Level 4 products, considered ultra-low bleed, are crucial for applications requiring the cleanest testing environment, such as detecting trace pollutants or conducting drug analyses. These advanced septa not only enhance test reliability but also help labs meet strict regulatory standards, such as those from the EPA or FDA.

Interested in our products? Contact us here to be connected to our sales team.